Design and optimize high multiplexing digital PCR assays with the naica® system

Digital PCR (dPCR) technologies are powerful tools that can be adapted to a variety of specialized applications. This talk explores target multiplexing in qPCR and dPCR assays to maximize the potential of today’s PCR instruments. In this webinar, we discuss critical aspects for dye selection, address inter-assay dimer concerns, and compare singleplex with multiplex data to ensure assay compatibility. For more challenging targets, including multiplexing allelic discrimination assays, we look closer at the advantages of IDT's Affinity Plus™ locked nucleic acid probes over conventional hydrolysis probes. The second part of the webinar focuses on highly multiplexed dPCR assays using the new Stilla® naica® system for Crystal Digital PCR™. The naica® system is a complete digital PCR solution with flexible sample throughput and highly sensitive detection and quantification of target nucleic acids. The new Prism fluorescent scanner allows target detection in up to 6-color channels, enabling another exclusive dimension for multiplexed dPCR assays. We also present data explaining SNP detection in liquid biopsy samples obtained using the naica dPCR instrument.